Inside structures acrobat ants typically nest in wood which has been subjected to high moisture and fungal decay, the same wood conditions favored by carpenter ants. Similarly, they will nest in Styrofoam insulation panels and in wall voids. Outside most species nest under rocks, or in logs, firewood, or trees where decay enables them to tunnel under the bark and/or into the wood. They occasionally will nest in abandoned termite and carpenter ant galleries as well as in old weed borer, powder post beetle, or wood wasp tunnels.
The workers readily enter structures by trailing along tree limbs and utility lines as well as along the rails of connected fences and decks. They then enter via cracks and holes around utility lines/pipes, window frames, soffits, etc. Workers also will trail across the ground and enter via door thresholds, weep holes, and other openings or cracks. They have been found to trail over 100 feet.
When disturbed or alarmed, workers of all but the smallest colonies tend to be quite aggressive. They are quick to bite, and give off a repulsive odor.